Xf setup
Author: l | 2025-04-24
The SETUP screen for Standalone operation is slightly different from the SETUP screen for VST use (Both are shown below) Double click the Motif XF icon and the Motif XF VST graphic interface opens: Click on FILE SETUP on the Motif XF Editor toolbar. Here you want to setup the communication between the Motif XF and the Editor.
Mic Setup (Motif XF) - YouTube
Measure oxygen and proton flux of cells grown in XF cell culture plates. The analyzer is used in conjunction with XF sensor cartridges. - XF Controller - Operation of the Analyzer is done through a high-resolution color LCD touch screen with stand, that may be installed in front of or beside the XF Pro. The controller communicates with the analyzer using a single serial cable and a single USB A-B cable. Suitable locations for the XF Pro system XF Pro Analyzers are designed for laboratory use. The internal environment of the analyzer is controlled to a preset temperature by the user; therefore, laboratory room temperature must be maintained within the range listed in the specification table. Sample temperature control performance can be monitored using the status display on the right side of the analyzer or on the Wave application display. The XF Pro uses optical detection technology to measure extremely low levels of fluorescent emission from analyte sensors. While the analyzer has been designed to shield room light, excessive light (such as direct sunlight) should be avoided. WARNING The electrical connection at the back of the XF Pro is the primary disconnect for the instrument. The XF Pro should be positioned to allow accessibility to the power cord for easy disconnection. CAUTION Avoid drafty areas, as well as areas experiencing significant vibration (such as a centrifuge). 18 Seahorse XF Pro Analyzer Operating Manual Installation 2 Internal components of the XF Pro Analyzer Internal components of the XF Pro Analyzer Removing the side doors reveals the measurement chamber in which the assay is conducted. The electro-optics hardware is enclosed in a card cage in the rear chamber, and this is connected to the probe head through a set of fiber optic cable bundles. The base of the enclosure contains the primary controller board and heater assembly. (See Figure 1 and Figure 2.) - LCD message screen - Displays current instrument action and setpoint temperature, and sample temperature. - Cover latches - Pull on indented hand-holds molded into the side doors (not shown) to lift them up, exposing the internal components of the instrument. Magnets hold in place. - Probe head and injection manifold - The probe head consists of 96 "light guides" to carry the optical signals to and from the sensors. The injection manifold uses compressed air to inject compounds loaded into sensor cartridge ports into the assay wells. Figure 1 XF Pro front/side view. Base color may vary. Fiberoptics Injection manifold Thermal tray Manifold heater LCD screen Cover latches (not shown) Seahorse XF Pro Analyzer Operating Manual 19 2 Installation Setup and interconnects: cable installation - Barcode reader - Reads barcode on sensor cartridge and cell plate. Setup and interconnects: cable installation The XF Pro Analyzer is operated from a touch screen computer monitor mounted to a stand, referred to as a controller. One RS232 cable and one USB cable handle the communication of commands and data between the instrument and the controller. The controller may be connected to
Motif XF Setup Templates for Cubase - YamahaSynth
News |Phase One firmware updates XF system, promises unlimited shutter actuationss News You Might Also Like… Phase One has introduced a new firmware update to its medium format XF Camera System that adds Electronic Shutter integration, among other new features.Available now and free for all Phase One XF camera system owners, the new firmware update includes complete Electronic Shutter integration on the IQ3 100-megapixel system.And Phase One has extended its warranty on the XF Camera System to include unlimited shutter actuations.What’s more, the new Phase One firmware update adds enhancements for studio and flash photography with the in-camera Profoto Air Remote enabling Profoto TTL metering.The update also adds a post-capture Flash Analysis Tool to give even more control over the lighting. Full functionality of the Profoto Air for up to six groups is now built directly into the XF Camera System.Phase One has also announced a new XF V-Grip for the system and added new customisation options such as Icon Control, Create, Load & Save System Setup and Advanced Tool Options for sequence tools.Click here to download the new Phase One XF firmware update.The new XF V-Grip price tag will be $1,490 and will be released in November 2016.READ MOREHands-on Fuji GFX 50S review Hands-on Olympus OM-D E-M1 Mark II review Cameras Firmware Update Medium Format Cameras Stay connected Sign-up to receive our newsletter and information about Camera Jabber Photographer of the Year --> --> --> Camera Jabber Jabber Digital Media Ltd. - 4 Beau Street, Bath, BA1 1QY. All rights reserved.12 xf 800 track/gear setup
Be securely attached and plate tray door is closed. This also prevents heat loss and system cooling which can affect data quality. 22 Seahorse XF Pro Analyzer Operating Manual 3 Basic Operation Power and Warm Up 24 Power up 24 Launch Wave 24 Performing XF assays 25 XF Pro status indicator 25 Wave Controller widgets 25 XF Pro Assays at Non-37 C Temperatures 28 Operational and assay guidelines for non-37 C assays 28 Set alarm (temperature tolerance range) 30 This chapter provides basic operating procedures for the Agilent Seahorse XF Pro Analyzer. Seahorse XF Pro Analyzer Operating Manual 23 3 Basic Operation Power and Warm Up Power and Warm Up Power up To power on the touch screen controller, press the power switch on the front of the controller. To prevent accidental power down of the controller, the switch may be disengaged in the Power Options menu of the Windows OS control panel under the Advanced tab. Turn on the instrument using the power switch on the rear panel. On the right side of the instrument near the access door, there is an LCD message screen. When the instrument is powered up the LCD message screen will show WAITING FOR WAVE. (See Figure 6.) Launch Wave When Wave software is launched on the controller, the LCD message screen will update and show the following display in Figure 7. Figure 6 LCD message screen (idle). Figure 7 LCD message screen (ready). 24 Seahorse XF Pro Analyzer Operating Manual Basic Operation 3 Performing XF assays Performing XF assays Information and protocols for preparing medium associated with XF assays, experimental design, running XF assays and analyzing XF data may be found online at www.agilent.com/en/product/cell-analysis/how-to-run-an-assay. XF Pro status indicator During an assay, the Status Indicator light on the top of the XF Pro Analyzer will change from green to amber if a task requires user interaction, such as: - Waiting for Wave (not connected to Wave or controller). - To load a sensor cartridge or cell plate. - To remove a used sensor cartridge or cell plate. - To accept or cancel an assay if one or more wells did not calibrate properly after calibration. - User interaction timeout. - Cover not properly attached or tray door left open. The Status Indicator light will change from green to red if an error has occurred, such as: - Any errors that can occur during the run, such as barcode read errors for the sensor cartridge, cell plate, or a protocol error. - Cannot connect to the barcode. - Motion error/motor stall. - Other instrument errors. Wave Controller widgets The widget icons are located on the lower left side of the XF Pro Controller software and display the status of the XF Pro Analyzer and current temperature. NOTE Allow at least 1 to 2 hours (or overnight) for the instrument to fully warm and equilibrate to the set temperature. If starting ambient temperature conditions are Seahorse XF Pro Analyzer Operating Manual 25 3 Basic Operation XF. The SETUP screen for Standalone operation is slightly different from the SETUP screen for VST use (Both are shown below) Double click the Motif XF icon and the Motif XF VST graphic interface opens: Click on FILE SETUP on the Motif XF Editor toolbar. Here you want to setup the communication between the Motif XF and the Editor. The SETUP screen for STAND ALONE operation is slightly different from the SETUP screen for VST use. (Both are shown below) Double click the Motif XF icon and the Motif XF VST graphic interface opens: Click on FILE SETUP on the Motif XF Editor toolbar Here you want to setup the communication between the Motif XF and the Editor.Saving Custom setups on the XF camera system
Contact, and additional resource information for the Agilent Seahorse XF Pro Analyzer. Seahorse XF Pro Analyzer Operating Manual 3 This page intentionally left blank. 4 Seahorse XF Pro Analyzer Operating Manual Contents About This Manual 3 1 Introduction General Information 8 Safety Considerations 9 Electrical hazards 9 Safety Labels 10 Instrument Overview and Intended Use 11 Technical Specifications 12 2 Installation Unpacking and Component Identification 14 Installation Procedure 18 Suitable locations for the XF Pro system 18 Internal components of the XF Pro Analyzer 19 Setup and interconnects: cable installation 20 3 Basic Operation Power and Warm Up 24 Power up 24 Launch Wave 24 Performing XF assays 25 XF Pro status indicator 25 Wave Controller widgets 25 XF Pro maintenance 26 XF Pro Assays at Non-37 C Temperatures 28 Operational and assay guidelines for non-37 C assays 28 Set alarm (temperature tolerance range) 30 4 Maintenance Cleaning and Routine Maintenance 32 Seahorse XF Pro Analyzer Operating Manual 5 Troubleshooting 33 Barcode errors 33 Contact Information 36 Worldwide technical support 36 Ordering 36 Additional Resources 37 6 Seahorse XF Pro Analyzer Operating Manual 1 Introduction General Information 8 Safety Considerations 9 Instrument Overview and Intended Use 11 Technical Specifications 12 This chapter provides an introduction to the Agilent Seahorse XF Pro Analyzer. Seahorse XF Pro Analyzer Operating Manual 7 1 Introduction General Information General Information This manual covers all models of Agilent Seahorse XF Pro Analyzer: Instrument type Part Number(s) Seahorse XF Pro S7850A (manufacturing part reference #S7850-64000) 8 Seahorse XF Pro Analyzer Operating Manual Introduction 1 Safety Considerations Safety Considerations The XF Pro has been carefully designed so that when used properly the instrument is accurate, fast, flexible, and safe. Information on safety practices is provided with the instrument and operation manuals. Before using the instrument or accessories, thoroughly read these safety practices. Electrical hazards Panels or covers that are retained by fasteners which require the use of a tool for removal may be opened only by Agilent-trained, -qualified, or authorized service engineers. Consult the manuals or product labels supplied with the XF Pro to determine which parts are operator-accessible. Instrument must always be turned off and disconnected from all power before removing any outer covers. Replace all covers prior to reconnecting power. Always use a three-wire outlet with ground connection which is adequately rated for the load. The installation must comply with local safety regulations. Always use the supplied power cords. Use of power cord with inadequate ratings can create a fire hazard or cause damage to the instrument. WARNING Observe all relevant safety practices at all times. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Unskilled, improper, or careless use of this instrument can create shock hazards, fire hazards, or other hazards which can cause death, serious injury to personnel, or severe damage to equipment and property. WARNING The XF Pro contains electrical circuits, devices, and components operating at dangerous voltages. Contact withMotif XF YSFW Setup with Cubase on Windows
An external network, through the ports on the underside. See Unpacking and Component Identification on page 14 to identify each cord, and refer to Figures 3 and 4 to identify the connectors. 1 Connect power cords - One power cord is used to connect the instrument to a grounded AC (mains) outlet. A second power cord is used to connect the controller power supply module to the AC supply. The power supply module is Figure 2 XF Pro front view. Barcode reader 20 Seahorse XF Pro Analyzer Operating Manual Installation 2 Setup and interconnects: cable installation then connected to the socket at the bottom of the controller. (See Figure 3.) 2 Connect the data cables to the analyzer. One RS-232 cable connects the controller serial port to the analyzer socket labeled "COM". (See Figure 4.) 3 A second cable (USB) connects the analyzer socket labeled "USB" to the USB port on the controller. This port must be used for proper functioning of the instrument and barcode reader. (See Figure 3.) 4 Connect external network cables. The controller may be networked via the Ethernet port on the controller. 5 Connect the AC power cord to the AC input on the XFe rear panel and then switch the power switch to the on position. (See Figure 5.) Figure 3 Controller ports (underside). Figure 4 XF Pro rear panel - USB and serial ports. USB RS-232 Seahorse XF Pro Analyzer Operating Manual 21 2 Installation Setup and interconnects: cable installation 6 Securely attach the cover and close the tray door. When the cover is securely attached and tray door is closed, optical switches are engaged to monitor the system. An optical sensor is also used to determine the status of the door. The XF Pro has a heater that maintains a stable internal system temperature. Typically, the temperature will be maintained at 37 C, as monitored by temperature sensors and controllers embedded in the tray and above the tray. A thermal fuse will disable the sample tray heater should it reach an abnormally high temperature. The manifold heater will not be disabled if this situation occurs, and the user should power off the instrument and contact technical support. In this situation the user should not attempt to open the instrument covers. Figure 5 XF Pro rear panel - AC input and power switch. Power switch I: on, O: off AC input WARNING The door opens automatically when the tray is extended, allowing the operator to insert or remove the well plate/cartridge consumables. The operator must exercise caution during the loading of the well plate/cartridge to avoid the possibility of a pinch hazard. After the well plate/cartridge is securely on the tray, the operator's hand must be removed from the area of the tray before continuing the assay. After the command is given to continue the assay via the controller, the tray will move slowly back into the instrument and the door will close. CAUTION Safe operation of the instrument requires that the coverStudioRIP XF Tutorial - Setup Calibration of Epson - YouTube
Fujifilm XF 27mm F2.8 R WR Fujifilm XF 30mm F2.8 R LM WR Fujifilm XF 33mm F1.4 R LM WR Fujifilm XF 35mm F1.4 R Fujifilm XF 35mm F2 R WR Fujifilm XF 500mm F5.6 R LM OIS WR Fujifilm XF 50-140mm F2.8 R LM OIS WR Fujifilm XF 50mm F1.0 R WR Fujifilm XF 50mm F2 R WR Fujifilm XF 55-200mm F3.5-4.8 R LM OIS Fujifilm XF 56mm F1.2 R Fujifilm XF 56mm F1.2 R APD Fujifilm XF 56mm F1.2 R WR Fujifilm XF 60mm F2.4 R Macro Fujifilm XF 70-300 F4-5.6 R LM OIS WR Fujifilm XF 80mm F2.8 R LM OIS WR Macro Fujifilm XF 8-16mm F2.8 R LM WR Fujifilm XF 8mm F3.5 R WR Fujifilm XF 90mm F2 R LM WR Hartblei Superrotator 40mm F4 IF TS Hartblei Superrotator 80mm F2.8 IF TS Hartblei Superrotator Macro 120mm F4 TS Hasselblad XCD 120mm F3.5 Macro Hasselblad XCD 135mm F2.8 Hasselblad XCD 20-35mm F3.2-4.5 E Hasselblad XCD 21mm F4 Hasselblad XCD 25mm F2.5 V Hasselblad XCD 28mm F4 P Hasselblad XCD 30mm F3.5 Hasselblad XCD 35-75mm F3.5-4.5 Hasselblad XCD 38mm F2.5 V Hasselblad XCD 45mm F3.5 Hasselblad XCD 45mm F4 P Hasselblad XCD 55mm F2.5 V Hasselblad XCD 65mm F2.8 Hasselblad XCD 75mm F3.4 P Hasselblad XCD 80mm F1.9 Hasselblad XCD 90mm F2.5 V Hasselblad XCD 90mm F3.2 HD Pentax DA 55-300mm F4.0-5.8 ED WR HD Pentax D FA 24-70mm F2.8 ED SDM WR HD Pentax D FA 645 Macro 90mm F2.8 ED AW SR HD Pentax DA 15mm F4 ED AL Limited HD Pentax DA 16-85mm F3.5-5.6 ED DC WR HD Pentax DA 21mm F3.2 AL Limited HD Pentax DA 35mm F2.8 Macro Limited HD Pentax DA 40mm F2.8 Limited HD Pentax DA 70mm F2.4 AL Limited HD Pentax DA AF 1.4X AW Rear Converter HD PENTAX-D FA 15-30mm F2.8 ED SDM WR HD PENTAX-D FA 21mm F2.4ED Limited DC WR HD PENTAX-D FA 28-105mm F3.5-5.6 ED DC WR HD Pentax-D FA 70-210mm F4 ED SDM WR HD PENTAX-D FA MACRO 100mm F2.8ED AW HD Pentax-D FA* 50mm F1.4 SDM AW HD Pentax-D FA* 85mm F1.4 ED SDM AW HD Pentax-D FA645 35mm F3.5 AL [IF] HD Pentax-DA 10-17mm F3.5-4.5 ED fisheye HD Pentax-DA 20-40mm F2.8-4 ED Limited DC WR HD Pentax-DA 55-300mm F4.5-6.3 ED PLM WR RE HD Pentax-DA* 11-18mm F2.8 HD Pentax-DA* 16-50mm F2.8 ED PLM AW HD Pentax-DA645 28-45mm F4.5ED AW SR HDMOTIF XF USB-MIDI Setup Windows - YamahaSynth
These circuits, devices, and components can cause death, serious injury, or painful electric shock. WARNING Connection of the instrument to an incorrectly wired supply outlet, or lack of proper electrical grounding can create a fire hazard or a potentially serious shock hazard and could seriously damage the instrument and any attached ancillary equipment. Seahorse XF Pro Analyzer Operating Manual 9 1 Introduction Safety Labels Safety Labels The following table lists the common safety labels you might find on the Agilent Seahorse XF Pro Symbol Description Indicates pinch, crush, or cut hazard. 10 Seahorse XF Pro Analyzer Operating Manual Introduction 1 Instrument Overview and Intended Use Instrument Overview and Intended Use The Agilent Seahorse XF Pro analyzer measures the rate of change of dissolved oxygen and pH in the media immediately surrounding living cells cultured in a microplate. Changes in the extracellular media are caused by the consumption or production of analytes by the cells. Therefore, a sensitive measurement of the media flux can be used to determine rates of cellular metabolism with great sensitivity and in a totally noninvasive, label-free manner. A unique feature of the Seahorse XF technology is its ability to make accurate and repeatable measurements in as little as five minutes. The instrument, working with a sensor cartridge, isolates a few L of media above the cell monolayer. Cellular metabolism causes rapid, easily measured changes to the "microenvironment" in this small volume. An XF sensor cartridge is required to run an assay. The cartridge has 96 probes, and each probe has a single multifluor sensor spot that is sensitive to both oxygen and proton concentration. The system measures the concentration of each analyte over time and automatically calculates the oxygen consumption rate (OCR) and proton efflux rate (PER) - which is a quantitative measure of extracellular acidification rate (ECAR) - simultaneously in every well of the microplate. Typically, a measurement cycle is performed for 6 minutes. The media is gently mixed, the probe is positioned 200 m above the well bottom, and the analyte levels are measured. Baseline metabolic rates are typically measured three to four times and are reported in pmol/min for OCR, mpH/min for ECAR, and pmol/min for PER. Compound is added to the media and mixed, and then the post-treatment OCR and ECAR measurements are made and repeated. As cells shift metabolic pathways, the relationship between OCR and ECAR/PER changes. The XF Pro system, comprising a bench top analyzer and touch screen controller, is driven by Agilent Seahorse Wave Pro software. This software enables all aspects of Seahorse XF assays including assay setup, instrument control, and data analysis. Consumables are sold separately and include Agilent Seahorse XF FluxPaks (comprising sensor cartridges, cell plates, and calibrant) as well as a variety of assay kits, reagents, and media. XF sensor cartridges are specific for the instrument type and can be purchased exclusively from Agilent. Seahorse XF Pro Analyzer Operating Manual 11 1 Introduction Technical Specifications Technical Specifications Model Number S7850A Controller Dimensions: Width height depth 21". The SETUP screen for Standalone operation is slightly different from the SETUP screen for VST use (Both are shown below) Double click the Motif XF icon and the Motif XF VST graphic interface opens: Click on FILE SETUP on the Motif XF Editor toolbar. Here you want to setup the communication between the Motif XF and the Editor. The SETUP screen for STAND ALONE operation is slightly different from the SETUP screen for VST use. (Both are shown below) Double click the Motif XF icon and the Motif XF VST graphic interface opens: Click on FILE SETUP on the Motif XF Editor toolbar Here you want to setup the communication between the Motif XF and the Editor.
MOTIF XF YSFW Setup Windows with Cubase - YamahaSynth
Carl Zeiss Distagon T* 2,8/15 ZM Carl Zeiss Distagon T* 2,8/21 Carl Zeiss Distagon T* 2,8/25 Carl Zeiss Distagon T* 2/25 Carl Zeiss Distagon T* 2/28 Carl Zeiss Distagon T* 2/35 Carl Zeiss Distagon T* 3,5/18 Carl Zeiss Distagon T* 4/18 ZM Carl Zeiss Makro-Planar T* 2/100 Carl Zeiss Makro-Planar T* 50mm F2 Carl Zeiss Planar T* 1,4/50 Carl Zeiss Planar T* 1,4/85 Carl Zeiss Planar T* 2/50 ZM Carl Zeiss Sonnar T* 2/85 ZM Carl Zeiss Tele-Tessar T* 4/85 ZM Carl Zeiss Touit 1.8/32 Carl Zeiss Touit 2.8/12 Fujifilm Fujinon MK 18-55mm T2.9 Fujifilm Fujinon MK 50-135mm T2.9 Fujifilm Fujinon MKX 18-55mm T2.9 Fujifilm Fujinon MKX 50-135mm T2.9 Fujifilm GF 100-200mm F5.6 R LM OIS WR Fujifilm GF 110mm F2 R LM WR Fujifilm GF 110mm F5.6 T/S Macro Fujifilm GF 120mm F4 R LM OIS WR Macro Fujifilm GF 20-35mm F4 R WR Fujifilm GF 23mm F4 R LM WR Fujifilm GF 250mm F4 R LM OIS WR Fujifilm GF 30mm F3.5 R WR Fujifilm GF 30mm F5.6 T/S Fujifilm GF 32-64mm F4 R LM WR Fujifilm GF 35-70mm F4.5-5.6 WR Fujifilm GF 45-100mm F4 R LM OIS WR Fujifilm GF 45mm F2.8 R WR Fujifilm GF 500mm F5.6 R LM OIS WR Fujifilm GF 50mm F3.5 R LM WR Fujifilm GF 55mm F1.7R WR Fujifilm GF 63mm F2.8 R WR Fujifilm GF 80mm F1.7 R WR Fujifilm XC 15-45mm F3.5-5.6 OIS PZ Fujifilm XC 16-50mm F3.5-5.6 OIS Fujifilm XC 16-50mm F3.5-5.6 OIS II Fujifilm XC 35mm F2 Fujifilm XC 50-230mm F4.5-6.7 OIS Fujifilm XC 50-230mm F4.5-6.7 OIS II Fujifilm XF 100-400mm F4.5-5.6 R LM OIS WR Fujifilm XF 10-24mm F4 R OIS Fujifilm XF 10-24mm F4 R OIS WR Fujifilm XF 14mm F2.8 R Fujifilm XF 150-600mm F5.6-8 R LM OIS WR Fujifilm XF 16-50mm F2.8-4.8 R LM WR Fujifilm XF 16-55mm F2.8 R LM WR Fujifilm XF 16-55mm F2.8 R LM WR II Fujifilm XF 16-80mm F4 R OIS WR Fujifilm XF 16mm F1.4 R WR Fujifilm XF 16mm F2.8 R WR Fujifilm XF 18-120mm F4 LM PZ WR Fujifilm XF 18-135mm F3.5-5.6 R LM OIS WR Fujifilm XF 18-55mm F2.8-4 R LM OIS Fujifilm XF 18mm F1.4 R LM WR Fujifilm XF 18mm F2 R Fujifilm XF 200mm F2 R LM OIS WR Fujifilm XF 23mm F1.4 R Fujifilm XF 23mm F1.4 R LM WR Fujifilm XF 23mm F2 R WR Fujifilm XF 27mm F2.8Saving Custom setups on the XF Camera System - YouTube
18" 13" 54 cm 46 cm 33 cm Analyzer Dimensions: 16" 24" 17" 41 cm 61 cm 43 cm Weight Analyzer: 49 lbs/23 kg Controller: 22 lbs/9 kg Power requirements 100 to 240 V AC 50/60Hz Analyzer: 300W Controller: 3.2 A Power cord rating Three-wire (grounded) AC power cord rated 10 A or Power Fuse ratings 250 V/10 A time delay fuses Environmental conditions "Normal" Environmental conditions- indoor use, altitude to 2,000 m Room temperature range +40 F to 86 F (+4 C to 30 C) No direct sunlight Do not place directly under air conditioning vents. 20 to 80% relative humidity Sample temperature and environment Controlled to user-selected temperature between 16 C and 42C, but at least 8 C above ambient temp No gas or humidity control Software OS Windows 10 LTSC, 64-bit Data interface RS-232c 64-bit barcode reader (internal) TCP/IP (external) USB Type B Equipment class Class 1 (PE connected) Pollution degree 2 Installation (overvoltage) category II Mains supply voltage fluctuations +/- 10% 12 Seahorse XF Pro Analyzer Operating Manual 2 Installation Unpacking and Component Identification 14 Installation Procedure 18 This chapter provides unpacking and installation instructions for the Agilent Seahorse XF Pro Analyzer. Seahorse XF Pro Analyzer Operating Manual 13 2 Installation Unpacking and Component Identification Unpacking and Component Identification The XF Pro Analyzer system is packaged in two boxes. Upon receipt, immediately check each box for damage. Shipping damage must be reported to the transportation company and Agilent. See Contact Information on page 36. The analyzer is shipped with protection components that must be removed prior to use. Agilent recommends these components be removed by Agilent personnel during installation. WARNING The XF Pro Analyzer requires two people to lift and handle Each person should firmly grasp the base of the unit at opposite ends to each other. Use Occupational Safety and Health Administration standards for lifting techniques. CAUTION XF Pro instruments must be installed by trained Agilent personnel. 14 Seahorse XF Pro Analyzer Operating Manual Installation 2 Unpacking and Component Identification To prevent damage during shipping, the instrument is shipped with a cartridge loaded onto the probe head and lowered onto a plate on the tray. These items must be removed prior to running the first assay. Agilent personnel will remove these shipping protection components from the XF Pro Analyzer during installation. Instrument/ Component Quantity Image XF Pro Instrument 1 XF Controller 64-bit 1 Seahorse XF Pro Analyzer Operating Manual 15 2 Installation Unpacking and Component Identification Power cord (instrument) 1 Power cord 1 Power supply (controller) 1 RS232 cable 1 Instrument/ Component Quantity Image 16 Seahorse XF Pro Analyzer Operating Manual Installation 2 Unpacking and Component Identification External barcode reader (optional) 1 USB cable 1 USB extension cable 1 Instrument/ Component Quantity Image Seahorse XF Pro Analyzer Operating Manual 17 2 Installation Installation Procedure Installation Procedure The following items are included in an XF Pro system: - XF Pro Analyzer - The analyzer is a temperature-controlled instrument containing all optical and electronic measurement components to. The SETUP screen for Standalone operation is slightly different from the SETUP screen for VST use (Both are shown below) Double click the Motif XF icon and the Motif XF VST graphic interface opens: Click on FILE SETUP on the Motif XF Editor toolbar. Here you want to setup the communication between the Motif XF and the Editor.Motif XF USB-MIDI Setup Macintosh - YamahaSynth
Pro maintenance XF Pro maintenance The maintenance options (in the Diagnostic menu) allow for ejection/insertion of the instrument tray, and to raise/lower the probes. 1 Click the Diagnostic menu at the top left of the main ribbon. 2 Select Maintenance, the last option in the list. Tray control: Manually eject or insert tray, with or without a utility plate or cell culture plate. Probe control: Manually raise/lower the probes of the XF Pro Analyzer. Cartridge control: Manually load or unload a sensor cartridge. Temperature widget: Current tray temperature and heater status display. Status widget: Connection status between the XF Pro Controller (computer), Wave Pro Controller (software), and the XF Pro Analyzer. 26 Seahorse XF Pro Analyzer Operating Manual Basic Operation 3 XF Pro maintenance Seahorse XF Pro Analyzer Operating Manual 27 3 Basic Operation XF Pro Assays at Non-37 C Temperatures XF Pro Assays at Non-37 C Temperatures Seahorse XF Pro Analyzers have been validated to deliver desired target temperatures in the range of 16 to 42 C, provided the ambient room temperature is 8 to 20 C below the target temperature, and in the validated operational room temperature range of 4 to 30 C. To understand the relationship between the desired sample temperature and required ambient temperature, see the temperature chart in Figure 8. Operational and assay guidelines for non-37 C assays - For all non-37 C operation, the XF Pro Analyzer must equilibrate overnight in the required ambient temperature. Figure 8 Temperature chart. Sample Temperature [C] Am bi en t T em pe ra tu re [ C] 16 18 20 22 24 26 28 30 32 34 36 38 40 42 4 6 8 10 12 14 16 18 20 22 24 26 28 30 28 Seahorse XF Pro Analyzer Operating Manual Basic Operation 3 Operational and assay guidelines for non-37 C assays - If it is required to set up the XF Pro Analyzer in a cold room, avoid direct fan sources. - For all non-37 C operation, the tray heater must remain On. Do not turn the tray heater Off. - For assay temperatures below 30 C, hydrate the sensor cartridge in the dark at room temperature. - Prior to starting an assay, an additional 30 minutes of precalibration equilibration time has been added to ensure temperature stability. To adjust the Target Temperature (set point) using the up/down arrows, do the following: 1 Click the temperature widget. (See Figure 9.) The Tray Heater dialog box is displayed. (See Figure 10.) Figure 9 Temperature widget. Figure 10 Edit temperature settings (Tray Heater dialog box). Seahorse XF Pro Analyzer Operating Manual 29 3 Basic Operation Set alarm (temperature tolerance range) 2 Ensure the ambient conditions support the desired target temperature (8 to 20 C above ambient). (See the temperature chart shown in Figure 8 on page 28.) 3 Other temperature widget functions are: Turn the heater On/Off. Set the tolerance range for temperature fluctuation. If the temperature is above or below the acceptable tolerance range from theComments
Measure oxygen and proton flux of cells grown in XF cell culture plates. The analyzer is used in conjunction with XF sensor cartridges. - XF Controller - Operation of the Analyzer is done through a high-resolution color LCD touch screen with stand, that may be installed in front of or beside the XF Pro. The controller communicates with the analyzer using a single serial cable and a single USB A-B cable. Suitable locations for the XF Pro system XF Pro Analyzers are designed for laboratory use. The internal environment of the analyzer is controlled to a preset temperature by the user; therefore, laboratory room temperature must be maintained within the range listed in the specification table. Sample temperature control performance can be monitored using the status display on the right side of the analyzer or on the Wave application display. The XF Pro uses optical detection technology to measure extremely low levels of fluorescent emission from analyte sensors. While the analyzer has been designed to shield room light, excessive light (such as direct sunlight) should be avoided. WARNING The electrical connection at the back of the XF Pro is the primary disconnect for the instrument. The XF Pro should be positioned to allow accessibility to the power cord for easy disconnection. CAUTION Avoid drafty areas, as well as areas experiencing significant vibration (such as a centrifuge). 18 Seahorse XF Pro Analyzer Operating Manual Installation 2 Internal components of the XF Pro Analyzer Internal components of the XF Pro Analyzer Removing the side doors reveals the measurement chamber in which the assay is conducted. The electro-optics hardware is enclosed in a card cage in the rear chamber, and this is connected to the probe head through a set of fiber optic cable bundles. The base of the enclosure contains the primary controller board and heater assembly. (See Figure 1 and Figure 2.) - LCD message screen - Displays current instrument action and setpoint temperature, and sample temperature. - Cover latches - Pull on indented hand-holds molded into the side doors (not shown) to lift them up, exposing the internal components of the instrument. Magnets hold in place. - Probe head and injection manifold - The probe head consists of 96 "light guides" to carry the optical signals to and from the sensors. The injection manifold uses compressed air to inject compounds loaded into sensor cartridge ports into the assay wells. Figure 1 XF Pro front/side view. Base color may vary. Fiberoptics Injection manifold Thermal tray Manifold heater LCD screen Cover latches (not shown) Seahorse XF Pro Analyzer Operating Manual 19 2 Installation Setup and interconnects: cable installation - Barcode reader - Reads barcode on sensor cartridge and cell plate. Setup and interconnects: cable installation The XF Pro Analyzer is operated from a touch screen computer monitor mounted to a stand, referred to as a controller. One RS232 cable and one USB cable handle the communication of commands and data between the instrument and the controller. The controller may be connected to
2025-04-17News |Phase One firmware updates XF system, promises unlimited shutter actuationss News You Might Also Like… Phase One has introduced a new firmware update to its medium format XF Camera System that adds Electronic Shutter integration, among other new features.Available now and free for all Phase One XF camera system owners, the new firmware update includes complete Electronic Shutter integration on the IQ3 100-megapixel system.And Phase One has extended its warranty on the XF Camera System to include unlimited shutter actuations.What’s more, the new Phase One firmware update adds enhancements for studio and flash photography with the in-camera Profoto Air Remote enabling Profoto TTL metering.The update also adds a post-capture Flash Analysis Tool to give even more control over the lighting. Full functionality of the Profoto Air for up to six groups is now built directly into the XF Camera System.Phase One has also announced a new XF V-Grip for the system and added new customisation options such as Icon Control, Create, Load & Save System Setup and Advanced Tool Options for sequence tools.Click here to download the new Phase One XF firmware update.The new XF V-Grip price tag will be $1,490 and will be released in November 2016.READ MOREHands-on Fuji GFX 50S review Hands-on Olympus OM-D E-M1 Mark II review Cameras Firmware Update Medium Format Cameras Stay connected Sign-up to receive our newsletter and information about Camera Jabber Photographer of the Year --> --> --> Camera Jabber Jabber Digital Media Ltd. - 4 Beau Street, Bath, BA1 1QY. All rights reserved.
2025-04-16Contact, and additional resource information for the Agilent Seahorse XF Pro Analyzer. Seahorse XF Pro Analyzer Operating Manual 3 This page intentionally left blank. 4 Seahorse XF Pro Analyzer Operating Manual Contents About This Manual 3 1 Introduction General Information 8 Safety Considerations 9 Electrical hazards 9 Safety Labels 10 Instrument Overview and Intended Use 11 Technical Specifications 12 2 Installation Unpacking and Component Identification 14 Installation Procedure 18 Suitable locations for the XF Pro system 18 Internal components of the XF Pro Analyzer 19 Setup and interconnects: cable installation 20 3 Basic Operation Power and Warm Up 24 Power up 24 Launch Wave 24 Performing XF assays 25 XF Pro status indicator 25 Wave Controller widgets 25 XF Pro maintenance 26 XF Pro Assays at Non-37 C Temperatures 28 Operational and assay guidelines for non-37 C assays 28 Set alarm (temperature tolerance range) 30 4 Maintenance Cleaning and Routine Maintenance 32 Seahorse XF Pro Analyzer Operating Manual 5 Troubleshooting 33 Barcode errors 33 Contact Information 36 Worldwide technical support 36 Ordering 36 Additional Resources 37 6 Seahorse XF Pro Analyzer Operating Manual 1 Introduction General Information 8 Safety Considerations 9 Instrument Overview and Intended Use 11 Technical Specifications 12 This chapter provides an introduction to the Agilent Seahorse XF Pro Analyzer. Seahorse XF Pro Analyzer Operating Manual 7 1 Introduction General Information General Information This manual covers all models of Agilent Seahorse XF Pro Analyzer: Instrument type Part Number(s) Seahorse XF Pro S7850A (manufacturing part reference #S7850-64000) 8 Seahorse XF Pro Analyzer Operating Manual Introduction 1 Safety Considerations Safety Considerations The XF Pro has been carefully designed so that when used properly the instrument is accurate, fast, flexible, and safe. Information on safety practices is provided with the instrument and operation manuals. Before using the instrument or accessories, thoroughly read these safety practices. Electrical hazards Panels or covers that are retained by fasteners which require the use of a tool for removal may be opened only by Agilent-trained, -qualified, or authorized service engineers. Consult the manuals or product labels supplied with the XF Pro to determine which parts are operator-accessible. Instrument must always be turned off and disconnected from all power before removing any outer covers. Replace all covers prior to reconnecting power. Always use a three-wire outlet with ground connection which is adequately rated for the load. The installation must comply with local safety regulations. Always use the supplied power cords. Use of power cord with inadequate ratings can create a fire hazard or cause damage to the instrument. WARNING Observe all relevant safety practices at all times. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Unskilled, improper, or careless use of this instrument can create shock hazards, fire hazards, or other hazards which can cause death, serious injury to personnel, or severe damage to equipment and property. WARNING The XF Pro contains electrical circuits, devices, and components operating at dangerous voltages. Contact with
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