Arabidopsis motif scanner

Author: b | 2025-04-24

Arabidopsis Motif Scanner 64bit Laptop Installer.exe: : 59.4 MB: 0. Arabidopsis Motif Scanner 32bit Installer.exe: : 59.4 MB: 0. Arabidopsis Motif

Arabidopsis Motif Scanner - iris.cnr.it

Above results confirm that our newly constructed ‘Gene-deletor’ vector driven by LFY promoter can achieve certain exogenous gene elimination effect in Arabidopsis thaliana.Fig. 2Analysis of eliminating effect of ‘Gene-deletor’ vector pBIN19-LFY-FLP in Arabidopsis thaliana. a Transgenic Arabidopsis thaliana homozygous T3 root GUS staining. b GUS staining in the stem of transgenic Arabidopsis thaliana homozygous T3. c GUS staining in leaves of transgenic Arabidopsis thaliana homozygous T3. d GUS staining in fruit pods of transgenic Arabidopsis thaliana homozygous T3. e GUS staining of the fruits of transgenic Arabidopsis thaliana homozygous T3. f The calculated elimination efficiency of exogenous genes in the fruits of transgenic Arabidopsis thaliana homozygous T3. The above experiments were repeated several times. Representative experiments were photographed, and the eliminating efficiency of exogenous genes was calculatedFull size imageGeneration of transgenic banana plants and their molecular analysisIn order to further verify the feasibility of using ‘Gene-deletor’ vector in bananas, ECSs of banana were genetically transformed using A. tumefaciens harboring ‘Gene-deletor’ vector (pCAMBIA-LFY-polseed-FLP) (Fig. 1e). Three subcultures in fresh medium were performed once in 10 days (Fig. 3a). Three months from transformation and selection, whitish embryos emerged on embryo development medium supplemented with 100 mg/L Kanamycin (Fig. 3b). These embryos were later transferred onto germination medium to aid the emergence of first plantlets (Fig. 3c), after which the tiny buds were transferred to multiplication medium. Clusters of small plantlets and white buds were observed after 1 month of culture (Fig. 3d). Single plantlets were isolated and rooted on rooting medium supplemented

Arabidopsis Motif Scanner - oa.las.ac.cn

LFY promoter ligated to the large fragment after enzyme digestion, and then undergoing BamHI and SalI double enzyme digestion to verify whether the LFY promoter ligated with the vector successfully. d PCR positive identification after recombinant vector transformation of Agrobacterium tumefaciens. e Schematic diagram of the newly constructed ‘Gene-deletor’ vector pCAMBIA-LFY-polseed-FLPFull size imageVerification of the elimination effect of ‘Gene-deletor’ vector in Arabidopsis thalianaIn order to verify the elimination effect of ‘Gene-deletor’ vector exogenous gene driven by the newly constructed LFY promoter, we first conducted genetic transformation verification in A. thaliana. GUS staining was performed on the roots, stems, leaves, fruit clips and fruit tissues of transgenic Arabidopsis thaliana, and GUS activity was observed in the roots, stems, leaves and fruit clips (Fig. 2a–d), indicating that the newly constructed pCAMBIA-LFY-polseed-FLP vector could not achieve the effect of exogenous gene elimination in the roots, stems, leaves and anthers of A. thaliana. Keeping in mind that the LFY promoter is a key promoter affecting flowering and development of Arabidopsis, we hypothesize that the newly constructed ‘Gene-deletor’ vector might perform a certain eliminating function in late flowering bud differentiation phase (fruits) of plants. To test the above hypothesis, we further stained the transgenic Arabidopsis fruits with GUS, and found that of the 200 Arabidopsis fruits, only 23 showed positive GUS staining and 177 showed negative GUS staining (Fig. 2e). The results indicate that the exogenous genes in the non-stained Arabidopsis thaliana fruits were successfully eliminated, with an elimination effect of 88.5% (Fig. 2f). The

Arabidopsis Motif Scanner - core.ac.uk

After the initiation of vector elimination, but it could effectively remove exogenous genes in Arabidopsis thaliana fruits, with the eliminating effect reaching 88.5% (Fig. 2f).Since our newly constructed exogenous ‘Gene-deletor’ vector pCAMBIA-LFY-polseed-FLP has good exogenous gene eliminating effect in transgenic Arabidopsis thaliana fruits (Fig. 2), this discovery prompted us to study the feasibility of applying exogenous ‘Gene-deletor’ vector in banana fruits. In order to test our hypothesis, genetic transformation of Cavendish banana was carried out in this paper using the method reported earlier (Dou et al. 2016). The genetically transformed plants were successfully obtained through co-culture, screening, plant regeneration, rooting and other steps (Fig. 3). Five transgenic lines (#1, #5, #11, #13 and #17) were used for further analysis, along with the corresponding untransformed WT. GUS staining was performed on the transgenic banana fruits, and it was found that different levels of exogenous gene eliminating effects were obtained in different parts of the transgenic fruits (Fig. 5a). Luo et al. (2007) reported that after the elimination of exogenous genes, only about 100 bp of vector fragments were left in specific tissues. In this paper, specific primers were used to carry out PCR on the fruits of five transgenic banana lines, and it was found that no small fragments appeared in two lines (line 5 and line 17), indicating that exogenous gene elimination was not completed in the transgenic fruits. Small fragments of about 100 bp appeared in three transgenic lines (line 1, line 11 and line 13), however, the large. Arabidopsis Motif Scanner 64bit Laptop Installer.exe: : 59.4 MB: 0. Arabidopsis Motif Scanner 32bit Installer.exe: : 59.4 MB: 0. Arabidopsis Motif

Arabidopsis Motif Scanner - ouci.dntb.gov.ua

EmbroideryStudio Add-ons Create and reuse patterns for runs & fills Once you’ve created or digitized a patterned fill you like, e.g. repeated hearts, diamonds, etc, store it away as a Motif and use it whenever you want over and over again. Any pattern you create can be saved as a Motif Create repeating, decorative outlines using any Motif set 3D Warp works with Motif Fills to create 3D effects Simple to Create and Manage Any pattern you create or open from a file can be saved as a Motif. You can create Motif sets (for instance, diamonds or petals) and save your Motifs to any of them—one Motif can be copied from one set to another. Own an older version of EmbroideryStudio? Compatible with EmbroideryStudio Designing Includes Motif Fills, Runs, Singles 3D Warp Create Motif What's included in Motifs Motif Run Create Motif repeats along a digitized line. Create repeating, decorative outlines using any Motif from any Motif set. Control rotation angle, orientation and space. Use control points or enter precise horizontal and vertical dimensions from a selection menu to control scaling. When overlaps occur as two Motifs meet, you can remove the overlapping element from one of the Motifs. Motif Fills EmbroideryStudio Motif Fills automatically repeats the Motif in parallel rows to fill the shape. You’re in control of scale, spacing, stitch angle, rotation and more. Select different Motifs for forward and backward rows, or use dual Motifs: overlaying and offsetting, for instance, diamonds and stars. Dual Motifs can

Arabidopsis Motif Scanner Tabs. a Motif Scanner Tab. b

AbstractBanana (Musa spp.) is an important tropical crop. Banana industry is under biotic and abiotic stresses such as Fusarium wilt, typhoon, cold stress. Genetic engineering offers a powerful strategy to create germplasm of banana with enhanced resistance. The safety of genetically modified organisms has become a bottleneck restricting the popularization and application of genetically modified technology. In this study, a candidate promoter, LEAFY (LFY) for expression and flower initiation in Arabidopsis, was cloned and constructed to ‘Gene-deletor’ vector. Histochemical β-glucuronidase (GUS) staining results showed that the ‘Gene-deletor’ vector driven by LFY promoter could lead to 88.5% excision efficiency from Arabidopsis seeds based on more than 200 T3 progeny examined per event. GUS staining was found to be partially negative in transgenic bananas, however, polymerase chain reaction could still detect the presence of large fragments of the vector. These results suggest that although LFY promoter could not completely drive the ‘Gene-deletor’ vector to achieve the effect of complete elimination of exogenous gene in bananas, its efficiency of eliminating exogenous gene laid a theoretical foundation for cloning banana fruit-specific promoters, that is, ‘non-transgenic’ GM bananas.Key messageThese results suggest that LFY promoter could not completely drive the ‘Gene-deletor’ vector to achieve the effect of complete elimination of exogenous gene in bananas. Nevertheless, a certain effect of exogenous gene elimination laid a theoretical foundation for the next step of screening banana fruit-specific promoters, removing all exogenous genes from banana fruits, and solving the food safety problem of genetically modified bananas. Similar content being viewed

Download Arabidopsis Motif Scanner 2.1

University of Connecticut developed the ‘Gene-deletor’ technology. This technique uses the pollen and seed-specific PAB5 promoters, which automatically eliminate all foreign genes in the pollen and seeds before and after transgenic flowering. This solves the problem of genetic flow of transgenic plants, and solves the concern about the safety of genetically modified food (Li et al. 2007). The efficacy of this technique has been demonstrated in tobacco (Luo et al. 2007).Recently, Chong-Perez et al. (2013) reported that the feasibility of using developmentally controlled promoters to mediate marker excision in banana. Considering that the LEAFY gene is the key gene affecting plant flowering development, in order to complete the removal of exogenous genes in early fruit formation, in this study the LFY promoter of the flower-determination gene LEAFY from A. thaliana genome was cloned (Fig. 1a), and successfully replaced the PAB5 promoter in the exogenous ‘Gene-deletor’ vector pCAMBIA-PAB-polseed-FLP (Fig. 1b). In order to further verify the eliminating effect of the newly constructed exogenous gene vector pCAMBIA-LFY-polseed-FLP in fruits, we first carried out genetic transformation in A. thaliana. Through GUS staining of the roots, stems, leaves, fruit clip and fruits of transgenic T3 homozygous plants, it was found that only 23 of the 200 Arabidopsis fruits were stained, and 177 Arabidopsis fruits showed no GUS signal, while GUS gene activity was detected in roots, stems, leaves and fruit clips (Fig. 2a–e). These results indicated that the specific promoter LFY in A. thaliana could not remove exogenous genes in roots, stems and leaves. Arabidopsis Motif Scanner 64bit Laptop Installer.exe: : 59.4 MB: 0. Arabidopsis Motif Scanner 32bit Installer.exe: : 59.4 MB: 0. Arabidopsis Motif